Based on the GB/T24559 marine propeller anemometer test methods and test points, the propeller anemometer was tested in the lowspeed wind tunnel. According to the JJF1059.12012 measurement uncertainty evaluation and expression requirements, seven calibration points of wind speed were tested repeatedly, and the range method was used to evaluate the type A uncertainty. The sources of type B uncertainty in the testing process are analyzed and the type B uncertainty is evaluated. The results show that it is feasible to calibrate the propeller anemometer in the WZ860070E wind tunnel. At the same time, each test point above 2 m/s can meet the three times of the quantitative transmission relationship.